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vegfc  (R&D Systems)


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    Structured Review

    R&D Systems vegfc
    Vegfc, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vegf+c/Recombinant+Human+VEGF-C+(Cys156Ser)+Protein/pm41944190-231-16-17
    Average 94 stars, based on 41 article reviews
    vegfc - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Recombinant:

    Article Title: A 3D Microfluidic Model of Initial Lymphatic Vessels for Investigating Dendritic Cell Chemotaxis Under Inflammatory Condition
    Article Snippet: The cocktail consists of vascular endothelial growth factor-A (VEGF-A; R&D Systems). .. VEGF-C (R&D Systems), basic fibroblast growth factor (bFGF; Invitrogen), sphingosine1-phosphate (S1P; Sigma), and recombinant human endocan (ESM-1; R&D Systems). ..

    Cell Culture:

    Article Title: Arterial-Lymphatic-Like Endothelial Cells Appear in Hereditary Hemorrhagic Telangiectasia 2 and Contribute to Vascular Leakage and Arteriovenous Malformations
    Article Snippet: 1 Correspondence to: Yucheng Yao, MD, PhD, Division of Cardiology, David Geffen School of Medicine at UCLA, Box 951679, Los Angeles, CA 90095-1679, Email yyao@mednet.ucla.edu, or Kristina I. Boström, MD, PhD, Division of Cardiology, David Geffen School of Medicine at UCLA, Box 951679, Los Angeles, CA 900951679, Email kbostrom@mednet.ucla.edu *Y. Yang, X. Wu, and Y. Zhao contributed equally.. Supplemental Material is available at https://www.ahajournals.org/doi/suppl/10.1161/CIRCULATIONAHA.124.070925.. For Sources of Funding and Disclosures, see page XXX.

    Transfection:

    Article Title: Arterial-Lymphatic-Like Endothelial Cells Appear in Hereditary Hemorrhagic Telangiectasia 2 and Contribute to Vascular Leakage and Arteriovenous Malformations
    Article Snippet: 1 Correspondence to: Yucheng Yao, MD, PhD, Division of Cardiology, David Geffen School of Medicine at UCLA, Box 951679, Los Angeles, CA 90095-1679, Email yyao@mednet.ucla.edu, or Kristina I. Boström, MD, PhD, Division of Cardiology, David Geffen School of Medicine at UCLA, Box 951679, Los Angeles, CA 900951679, Email kbostrom@mednet.ucla.edu *Y. Yang, X. Wu, and Y. Zhao contributed equally.. Supplemental Material is available at https://www.ahajournals.org/doi/suppl/10.1161/CIRCULATIONAHA.124.070925.. For Sources of Funding and Disclosures, see page XXX.

    Affinity Purification:

    Article Title: VEGF-A, -C, -D, VEGFR1, -2, -3, PDGF-BB and FGF-2 join forces to induce vascular and lymphatic angiogenesis during bone healing of hip implants
    Article Snippet: .. Antibodies were used within 5 months of arrival to our lab. VEGF-C, CA: anti-human monoclonal mouse IgG2 B antibody (Biotechne R&D Systems, cat.no.MAB752), DA: biotinylated antigen affinity-purified polyclonal goat IgG antibody (Biotechne R&D Systems, cat.no.BAF752), hRP (Peprotech, cat.no. ..

    Article Title: VEGF-A, -C, -D, VEGFR1, -2, -3, PDGF-BB and FGF-2 join forces to induce vascular and lymphatic angiogenesis during bone healing of hip implants
    Article Snippet: .. Antibodies were used within 5 months of arrival to our lab. VEGF-C, CA: anti-human monoclonal mouse IgG2B antibody (Biotechne R&D Systems, cat.no.MAB752), DA: biotinylated antigen affinity-purified polyclonal goat IgG antibody (Biotechne R&D Systems, cat.no.BAF752), hRP (Peprotech, cat.no. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Inflammatory cytokines regulate secretion of VEGF and chemokines by human conjunctival fibroblasts: Role in dysfunctional tear syndrome
    Article Snippet: .. Elisa kits for the analysis of VEGF-A, VEGF-C, sVEGF-R1, endostatin, thrombospondin-1, CXCL9 (MIG), CXCL10 (IP-10), CXCL11 (iTAC) were purchased from R&D Systems (Minneapolis, MN). ..



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    <t>PAX5</t> suppresses invasion and migration of GAC lymph node metastatic cells. A : CCK-8 assay measuring the viability of GAC lymph node metastatic cells. B and E: Scratch assay demonstrating cell migration; scale bar = 100 μm. C and F: Transwell assay assessing cell invasion; scale bar = 100 μm. D and F : Immunofluorescence analysis of PAX5 expression; scale bar = 50 μm. G : Quantitative PCR (qPCR) analysis of mRNA expression levels of PAX5, <t>VEGF-C,</t> PIK3, and AKT1. H : Western blot analysis of protein expression levels of PAX5, VEGF-C, AKT1, PI3K, MMP2, and Ki-67. “ns” indicates “not significant”; * indicates P < 0.05; ** indicates P < 0.01; *** indicates P < 0.001; **** indicates P < 0.0001
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    <t>PAX5</t> suppresses invasion and migration of GAC lymph node metastatic cells. A : CCK-8 assay measuring the viability of GAC lymph node metastatic cells. B and E: Scratch assay demonstrating cell migration; scale bar = 100 μm. C and F: Transwell assay assessing cell invasion; scale bar = 100 μm. D and F : Immunofluorescence analysis of PAX5 expression; scale bar = 50 μm. G : Quantitative PCR (qPCR) analysis of mRNA expression levels of PAX5, <t>VEGF-C,</t> PIK3, and AKT1. H : Western blot analysis of protein expression levels of PAX5, VEGF-C, AKT1, PI3K, MMP2, and Ki-67. “ns” indicates “not significant”; * indicates P < 0.05; ** indicates P < 0.01; *** indicates P < 0.001; **** indicates P < 0.0001
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    Image Search Results


    (A) Expression of KDR, encoding VEGFR2, and (B) FLT4, encoding VEGFR3, across publicly available pediatric solid tumor datasets included in the R2 MegaSampler platform. Expression values are presented as log2-transformed signal intensity. The distributions illustrate inter-dataset and inter-tumor heterogeneity in the expression of receptors associated predominantly with VEGF-A and VEGF-C signaling, respectively. M, metastasis; T, primary tumor; ES, Ewing sarcoma; NB, neuroblastoma; OS, osteosarcoma.

    Journal: bioRxiv

    Article Title: Computationally guided design of a metastasis-on-a-chip platform for quantitative evaluation of chemotactic cues in developmental cancers

    doi: 10.64898/2026.07.25.740695

    Figure Lengend Snippet: (A) Expression of KDR, encoding VEGFR2, and (B) FLT4, encoding VEGFR3, across publicly available pediatric solid tumor datasets included in the R2 MegaSampler platform. Expression values are presented as log2-transformed signal intensity. The distributions illustrate inter-dataset and inter-tumor heterogeneity in the expression of receptors associated predominantly with VEGF-A and VEGF-C signaling, respectively. M, metastasis; T, primary tumor; ES, Ewing sarcoma; NB, neuroblastoma; OS, osteosarcoma.

    Article Snippet: VEGF-A165 and VEGF-C concentrations were quantified using commercially available human ELISA kits (RayBiotech, Peachtree Corners, GA, USA; Catalog Nos.

    Techniques: Expressing, Transformation Assay

    VEGF-C transport was simulated using initial concentrations of 0.1 or 5 µg/mL in Chamber 2. Predicted VEGF-C concentrations over time are shown in (A) Channel 2, adjacent to the source, (B) at the microchannel array separating Reservoirs 2 and 1, and (C) in Channel 1, on the Chamber 1 side of the device. An initial concentration of 5 µg/mL generated measurable exposure at the microchannel array within the experimental time window, whereas 0.1 µg/mL produced substantially lower concentrations and negligible predicted exposure at the microchannel array and in Channel 1. Different concentration units and y-axis ranges were used for the individual device regions.

    Journal: bioRxiv

    Article Title: Computationally guided design of a metastasis-on-a-chip platform for quantitative evaluation of chemotactic cues in developmental cancers

    doi: 10.64898/2026.07.25.740695

    Figure Lengend Snippet: VEGF-C transport was simulated using initial concentrations of 0.1 or 5 µg/mL in Chamber 2. Predicted VEGF-C concentrations over time are shown in (A) Channel 2, adjacent to the source, (B) at the microchannel array separating Reservoirs 2 and 1, and (C) in Channel 1, on the Chamber 1 side of the device. An initial concentration of 5 µg/mL generated measurable exposure at the microchannel array within the experimental time window, whereas 0.1 µg/mL produced substantially lower concentrations and negligible predicted exposure at the microchannel array and in Channel 1. Different concentration units and y-axis ranges were used for the individual device regions.

    Article Snippet: VEGF-A165 and VEGF-C concentrations were quantified using commercially available human ELISA kits (RayBiotech, Peachtree Corners, GA, USA; Catalog Nos.

    Techniques: Concentration Assay, Generated, Produced

    Journal: bioRxiv

    Article Title: Computationally guided design of a metastasis-on-a-chip platform for quantitative evaluation of chemotactic cues in developmental cancers

    doi: 10.64898/2026.07.25.740695

    Figure Lengend Snippet:

    Article Snippet: VEGF-A165 and VEGF-C concentrations were quantified using commercially available human ELISA kits (RayBiotech, Peachtree Corners, GA, USA; Catalog Nos.

    Techniques: Incubation, Cell Culture, Enzyme-linked Immunosorbent Assay

    (A) Representative merged bright-field and fluorescence images of neuroblastoma, Ewing sarcoma, and osteosarcoma cells detected within the microchannel array under control, VEGF-A165, and VEGF-C conditions. Images were processed using a custom Fiji macro that automatically identified the microchannel region, detected fluorescent cells, and retained cells located within the microchannels according to predefined size, circularity, intensity, and channel-overlap criteria. Scale bars =50µm. (B) Quantification of the number of cells detected within the microchannels for neuroblastoma, Ewing sarcoma, and osteosarcoma. Individual data points represent independent microfluidic devices, and bars show mean ± SD. Statistical significance was assessed using ordinary one-way ANOVA followed by Dunnett’s multiple-comparisons test against the corresponding control. *p < 0.05; ns, not significant.

    Journal: bioRxiv

    Article Title: Computationally guided design of a metastasis-on-a-chip platform for quantitative evaluation of chemotactic cues in developmental cancers

    doi: 10.64898/2026.07.25.740695

    Figure Lengend Snippet: (A) Representative merged bright-field and fluorescence images of neuroblastoma, Ewing sarcoma, and osteosarcoma cells detected within the microchannel array under control, VEGF-A165, and VEGF-C conditions. Images were processed using a custom Fiji macro that automatically identified the microchannel region, detected fluorescent cells, and retained cells located within the microchannels according to predefined size, circularity, intensity, and channel-overlap criteria. Scale bars =50µm. (B) Quantification of the number of cells detected within the microchannels for neuroblastoma, Ewing sarcoma, and osteosarcoma. Individual data points represent independent microfluidic devices, and bars show mean ± SD. Statistical significance was assessed using ordinary one-way ANOVA followed by Dunnett’s multiple-comparisons test against the corresponding control. *p < 0.05; ns, not significant.

    Article Snippet: VEGF-A165 and VEGF-C concentrations were quantified using commercially available human ELISA kits (RayBiotech, Peachtree Corners, GA, USA; Catalog Nos.

    Techniques: Fluorescence, Control

    PAX5 suppresses invasion and migration of GAC lymph node metastatic cells. A : CCK-8 assay measuring the viability of GAC lymph node metastatic cells. B and E: Scratch assay demonstrating cell migration; scale bar = 100 μm. C and F: Transwell assay assessing cell invasion; scale bar = 100 μm. D and F : Immunofluorescence analysis of PAX5 expression; scale bar = 50 μm. G : Quantitative PCR (qPCR) analysis of mRNA expression levels of PAX5, VEGF-C, PIK3, and AKT1. H : Western blot analysis of protein expression levels of PAX5, VEGF-C, AKT1, PI3K, MMP2, and Ki-67. “ns” indicates “not significant”; * indicates P < 0.05; ** indicates P < 0.01; *** indicates P < 0.001; **** indicates P < 0.0001

    Journal: Journal of Translational Medicine

    Article Title: Single-cell and spatial transcriptomic analysis reveals PAX5’s role and regulatory mechanisms in gastric adenocarcinoma lymph node metastasis

    doi: 10.1186/s12967-026-08059-4

    Figure Lengend Snippet: PAX5 suppresses invasion and migration of GAC lymph node metastatic cells. A : CCK-8 assay measuring the viability of GAC lymph node metastatic cells. B and E: Scratch assay demonstrating cell migration; scale bar = 100 μm. C and F: Transwell assay assessing cell invasion; scale bar = 100 μm. D and F : Immunofluorescence analysis of PAX5 expression; scale bar = 50 μm. G : Quantitative PCR (qPCR) analysis of mRNA expression levels of PAX5, VEGF-C, PIK3, and AKT1. H : Western blot analysis of protein expression levels of PAX5, VEGF-C, AKT1, PI3K, MMP2, and Ki-67. “ns” indicates “not significant”; * indicates P < 0.05; ** indicates P < 0.01; *** indicates P < 0.001; **** indicates P < 0.0001

    Article Snippet: Antibody details are as follows: PAX5 (A12048, ABclonal, Wuhan, China), VEGF-C (A2556, ABclonal, Wuhan, China), AKT1 (A17909, ABclonal, Wuhan, China), PI3K (A19742, ABclonal, Wuhan, China), MMP2 (A19080, ABclonal, Wuhan, China), and Ki-67 (A16919, ABclonal, Wuhan, China).

    Techniques: Migration, CCK-8 Assay, Wound Healing Assay, Transwell Assay, Immunofluorescence, Expressing, Real-time Polymerase Chain Reaction, Western Blot

    PAX5 modulates HLEC cell tube formation in a co-culture system. A : Quantification of the number of nodes and total length of major master segments in tube-like structures. B : qPCR analysis of mRNA expression levels of PAX5, VEGF-C, PIK3, and AKT1 in HLEC cells. “ns” indicates “not significant”; * indicates P < 0.05; ** indicates P < 0.01; *** indicates P < 0.001; **** indicates P < 0.0001; scale bars = 100 μm

    Journal: Journal of Translational Medicine

    Article Title: Single-cell and spatial transcriptomic analysis reveals PAX5’s role and regulatory mechanisms in gastric adenocarcinoma lymph node metastasis

    doi: 10.1186/s12967-026-08059-4

    Figure Lengend Snippet: PAX5 modulates HLEC cell tube formation in a co-culture system. A : Quantification of the number of nodes and total length of major master segments in tube-like structures. B : qPCR analysis of mRNA expression levels of PAX5, VEGF-C, PIK3, and AKT1 in HLEC cells. “ns” indicates “not significant”; * indicates P < 0.05; ** indicates P < 0.01; *** indicates P < 0.001; **** indicates P < 0.0001; scale bars = 100 μm

    Article Snippet: Antibody details are as follows: PAX5 (A12048, ABclonal, Wuhan, China), VEGF-C (A2556, ABclonal, Wuhan, China), AKT1 (A17909, ABclonal, Wuhan, China), PI3K (A19742, ABclonal, Wuhan, China), MMP2 (A19080, ABclonal, Wuhan, China), and Ki-67 (A16919, ABclonal, Wuhan, China).

    Techniques: Co-Culture Assay, Expressing

    In vivo validation of PAX5 functional mechanisms. A : Hematoxylin and eosin (H&E) staining of mouse tumors. B : Immunohistochemical analysis of PAX5, Ki-67, and MMP2 expression in mouse tumors. C : qPCR analysis of mRNA expression levels of PAX5, PIK3, AKT1, and VEGF-C in mouse tumors. D : Western blot analysis of protein expression levels of PAX5, PI3K, AKT1, and VEGF-C in mouse tumors. * indicates P < 0.05; ** indicates P < 0.01; *** indicates P < 0.001; **** indicates P < 0.0001; scale bars = 50 μm

    Journal: Journal of Translational Medicine

    Article Title: Single-cell and spatial transcriptomic analysis reveals PAX5’s role and regulatory mechanisms in gastric adenocarcinoma lymph node metastasis

    doi: 10.1186/s12967-026-08059-4

    Figure Lengend Snippet: In vivo validation of PAX5 functional mechanisms. A : Hematoxylin and eosin (H&E) staining of mouse tumors. B : Immunohistochemical analysis of PAX5, Ki-67, and MMP2 expression in mouse tumors. C : qPCR analysis of mRNA expression levels of PAX5, PIK3, AKT1, and VEGF-C in mouse tumors. D : Western blot analysis of protein expression levels of PAX5, PI3K, AKT1, and VEGF-C in mouse tumors. * indicates P < 0.05; ** indicates P < 0.01; *** indicates P < 0.001; **** indicates P < 0.0001; scale bars = 50 μm

    Article Snippet: Antibody details are as follows: PAX5 (A12048, ABclonal, Wuhan, China), VEGF-C (A2556, ABclonal, Wuhan, China), AKT1 (A17909, ABclonal, Wuhan, China), PI3K (A19742, ABclonal, Wuhan, China), MMP2 (A19080, ABclonal, Wuhan, China), and Ki-67 (A16919, ABclonal, Wuhan, China).

    Techniques: In Vivo, Biomarker Discovery, Functional Assay, Staining, Immunohistochemical staining, Expressing, Western Blot